RUB researchers draw precise 3D chemical landscapes using non-invasive methods
Picturing infertility – potential to distinguish fertile and infertile human sperm cells
Bochum, 18.05.2010 – Scientists from Ruhr-Universität Bochum have developed a non-invasive technique that within seconds can distinguish healthy fertile and infertile sperm cells by collecting the spectral chemical fingerprint. The method has the potential for a novel fertility technology and a test scheme which does not only rely on morphological characteristics, but also utilizes chemical signatures. These findings are of fundamental significance and are thus featured as a Highlight in „Chemical Biology“ and in the News section of „Chemistry World” of the Royal Society of Chemistry in May.
Nature has optimized the main function of sperm cells in a similar way to race cars. These cells consist of sub-cellular organelles that contain, for example, mitochondria. Mitochondria are the power stations supplying energy for sperm motion and mobility; one of the crucial factors in fertility. The research team has shown that cellular damage on a molecular level in mitochondria can be present although changes in form and morphology are not visible. This stresses that besides morphology, which is a strict criterion by the WHO Manual for Andrology Laboratories to classify human sperm fertility, it is now possible and necessary to improve the test criteria by incorporating chemical signatures.
The RUB researchers obtained detailed 3D chemical maps which do not require the introduction of additional labels or markers. The Raman micro-spectroscopy used in this study detects sub-cellular components using the spectral fingerprints of molecules based on their characteristic vibrations. Different organelles within human sperm are visualized by their chemically unique Raman spectra. In addition to optical and morphological images, it is now possible to directly image the chemical constituents of individual human sperm cells.
This discovery may contribute to the development of new standards for the classification of healthy fertile and damaged infertile human sperm cells. Since the sperm count of human males around the world has dropped an alarming 50 per cent of what it was more than 50 years ago, there is an urgency to investigate human fertility organelles. The importance of this work was noted by the Royal Society of Chemistry and featured as a Highlight of “Chemical Biology” and as News in „Chemistry World”.
The development of innovative spectroscopic and microscopic methods for high resolution imaging of living cells is one of the research areas at the Chair of Physical Chemistry II (Prof. Dr. Martina Havenith-Newen). The work of Konrad Meister, Diedrich A. Schmidt and Erik Bründermann uses confocal Raman microscopy for detailed 3D imaging and identification of organelles of human sperm cells to reveal the origin of infertility on the molecular level. The funding for the Raman microscope used in this study was supported by the Federal Ministry of Education and Research (BMBF 05KS7PC2).
Title K. Meister , D. A. Schmidt , E. Bründermann, M. Havenith: Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa, Analyst (2010). DOI: 10.1039/b927012d
https://microscopy-news.com/wp-content/uploads/2017/01/516b76019f18322d8d3a9ddc96987741.jpg300350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:44:242017-01-17 10:44:24Andor launches KOMET 7 – The most advanced and powerful software solution for analysis, data management and presentation of comet assay samples
https://microscopy-news.com/wp-content/uploads/2017/01/543ba9c56fe57e856dc7f252e2ebd3ba.jpg466350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:43:462017-01-17 10:43:46Advanced Thin Films Enters Medical Laser Optics Market
https://microscopy-news.com/wp-content/uploads/2017/01/7423894303b540804bd4b9ded95ac7a1.jpg228350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:43:332017-01-17 10:43:33Module transforms Raman microscopes into high throughput chemical analyzers
https://microscopy-news.com/wp-content/uploads/2017/01/ca9ed333736eb38ce4abbffcee37be60.jpg260350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:42:462017-01-17 10:42:46Motic Microscopes launches the new BA410E for biomedical applications
https://microscopy-news.com/wp-content/uploads/2017/01/2ed54dfbb5613dc8894327c8185309c1.jpg175350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:42:442017-01-17 10:42:44R&D 100 Award 2014 for ZEISS ELYRA P.1 with 3D-PALM
https://microscopy-news.com/wp-content/uploads/2017/01/944a198042025732e6e8fb5a715d48c5.jpg243350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:41:572017-01-17 10:41:57Motic Microscopes presents the new AE31E for microbiology
https://microscopy-news.com/wp-content/uploads/2017/01/3725b94ba62d8e2205f5f20b3cdee1ee.jpg463350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:41:372017-01-17 10:41:37CRAIC Technologies Website: A Resource for UV-Visible-NIR and Raman Microspectroscopy
https://microscopy-news.com/wp-content/uploads/2017/01/1affabb625a5c37519bafa89fedc162e.jpg86350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:41:102017-01-17 10:41:10Imaging companies in Europe shoulder-to-shoulder with life scientists
https://microscopy-news.com/wp-content/uploads/2017/01/341633931c01418c4fa8e0a90a8236d4.jpg139350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:39:052017-01-17 10:39:05XIMEA unveils Thunderbolt™ 2 high speed cameras with IMX174 and CMV20000
https://microscopy-news.com/wp-content/uploads/2017/01/22b06d4094468fc910406cd44d96ec1f.jpg181350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:37:192017-01-17 10:37:19Dolomite´s new microfluidic system showcases droplet merging at nanoliter sample scale
https://microscopy-news.com/wp-content/uploads/2017/01/a5d0c77f7619812a129b2a4e6a9cb90b.jpg234350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:36:522017-01-17 10:36:52Point Grey´s Latest Blackfly® GigE PoE Camera Features Popular High Sensitivity VGA CCD
https://microscopy-news.com/wp-content/uploads/2017/01/a77db128b10a613c26b9e4dbea5dae02.jpg220350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:36:392017-01-17 10:36:39Point Grey Launches New Grasshopper®3 Family of High Resolution GigE Vision™ PoE Cameras