Promega Introduces Four New Proteases

Lyophilized mass spec protein sample prep reagents

12 January 2012, Madison, WI USA: Promega Corporation announced today that it has launched four new proteases, Pepsin, Elastase, Sequencing Grade Arg-C, and Thermolysin. These high quality proteases are the latest addition to Promega’s market leading range of protein sample prep reagents, which includes Trypsin Gold.

The new proteases are provided in a lyophilized format, enabling resuspension in any buffer. Applications include use in peptide mapping and protein identification experiments, and in characterization of post-translational modifications.

Pepsin preferentially cleaves at the C-terminus of phenylalanine, leucine, tyrosine and tryptophan. Elastase is a serine protease that has a unique capability of digesting elastin, preferentially cleaving at the C-terminus of alanine, valine, serine, glycine, leucine or isoleucine. Arg-C (clostripain) is a sequencing grade endopeptidase that cleaves at the C-terminus of arginine residues, including sites next to proline. Cleavage also will occur at lysine residues. These three enzymes may be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications.

Thermolysin is a thermostable metalloproteinase with optimal digestion temperature range of 65–85°C. High digestion temperatures may be used as an alternative to denaturants to improve digestion of proteolytically resistant proteins. Thermolysin preferentially cleaves at the N-terminus of the hydrophobic residues leucine, phenylalanine, valine, isoleucine, alanine and methionine.

For more information about Promega’s range of protein sample prep reagents please visit: www.promega.com/msprep

About Promega
Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company’s 2,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA, with branches in 15 countries and over 50 global distributors. For more information about Promega, visit: www.promega.com