PicoQuant´s 6th course on time-resolved microscopy and correlation spectroscopy in Berlin
Lectures and practical sessions covered FLIM, FLIM-FRET, FCS, FLCS and single molecule spectroscopy Berlin (Germany), 6 March 2014 – For the sixth time now, PicoQuant GmbH has organized the European Short Course on “Time-resolved Microscopy and Correlation Spectroscopy”, held in Berlin, Germany from February 25 to 27, 2014. The line-up of six leading experts gave an introduction into the basics of time-resolved fluorescence microscopy and also presented their latest research results to the 48 participants from all around the world. In the afternoons, practical sessions gave the participants the opportunity to use the cutting-edge equipment of PicoQuant, Nikon, Leica and Olympus.
Dr. Johan Hofkens, professor at the Department of Chemistry, KU Leuven, Belgium opened the course with a lecture about the basics of microscopy. He gave an overview of the different modes of optical and fluorescence microscopy and there applications including Stimulated Emission Depletion (STED) as a way to increase the resolution beyond the Abbé resolution limit.
His lecture was followed by basic instrumental requirements necessary to perform time-resolved fluorescence microscopy with special focus on excitation sources, detector options and electronics for Time-Correlated Single Photon Counting (TCSPC) by Rainer Erdmann, Managing Director of PicoQuant.
The second day started with a lecture of Dr. Mark Hink from the University of Amsterdam, The Netherlands, in which he explained the basics of Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Cross-Correlation Spectroscopy (FCCS). In the following lecture, Prof. Paul French from the Imperial College London, UK introduced the technique of Fluorescence Lifetime Imaging (FLIM) and showed applications in cell biology, drug discovery and clinical diagnosis.
The third day kicked off with a lecture of Dr. Ingo Gregor from the Georg-August-University, Göttingen, Germany. He explained advanced concepts of FCS such as Fluorescence Lifetime Correlation Spectroscopy (FLCS) and 2-focus FCS (2fFCS). In the second talk, then Prof. Fred S. Wouters from the University Medical Center of the Georg-August-University, Göttingen, Germany, gave an introduction to Förster Resonance Energy Transfer (FRET).
The lectures in the morning were complemented by practical sessions in the afternoon. Each experimental station focused on a specific time-resolved measurement method such as FLIM, FLIM-FRET, FCS, as well as single molecule experiments. The instruments presented included a time-resolved confocal microscope from PicoQuant and laser scannnig microscopes from Nikon, Leica and Olympus, each upgraded with time-resolved data acquisition capabilities using the dedicated kit from PicoQuant. One additional session was dedicated to data analysis using PicoQuant’s SymPhoTime software where participants had the chance to test all features of the software by analyzing previously measured data.
The participants especially liked the compact and comprehensive program, including the high quality of the lectures and instructive hands-on experiments. In summary, it was a memorable course bringing theory, instrumentation and various fields of application together.
PicoQuant GmbH is a research and development company in the field of optoelectronics. The company was founded in 1996 and is based in the science and technology park Berlin-Adlershof, Germany. The company is a worldwide leader in the field of single photon counting applications. The product line includes pulsed diode lasers and LEDs, photon counting instrumentation, fluorescence lifetime spectrometers and time-resolved confocal microscopes. PicoQuant employs currently around 60 people. Since April 2008 Sales and Support in North America is handled by PicoQuant Photonics North America Inc.
Practical_Session_TRMic_Course_2014.JPG Caption: Fluorescence Lifetime Imaging Microscopy (FLIM) at a practical session during PicoQuant’s microscopy course in February 2014.
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