Microscopy News
  • Microscopy News
    • Press Releases
    • Archive
    • Events/Workshops
    • Newsletter
  • Companies
    • Microscope and Accessiores
    • Imaging Software
    • Dealer
  • Products
    • Microscopes
      • Upright Microscopes
      • Inverted Microscopes
      • Stereo Microscopes
      • Electron Microscopes
    • Software
      • Imaging Software
    • Others
      • Imaging Cameras
      • Systems
      • Medical/Surgical Solutions
      • Accessoires
  • Download Center
    • Instructions
    • Tutorials
    • Brochures
    • Software
  • MediaKit / Sponsoring
  • Archive
  • Suche

Novel High-Resolution Methods in Fluorescence Microscopy

Heidelberg scientists employ chemical reaction for light-independent switching of fluorescent probes

Heidelberg, March 2, 2011 – Physical limits in high-resolution light microscopy can be overcome with the aid of chemical reactions. Scientists at Heidelberg University’s Institute of Physical Chemistry and members of the Cluster of Excellence “CellNetworks” have devised a new method in which light-dependent processes are replaced by chemical reactions to mark cellular structures for high-resolution optical microscopy. This method opens up new application vistas for fluorescence microscopy. The findings have been published online in the journal “Angewandte Chemie International Edition”.

Fluorescence microscopy is a widely used method for studying cellular structures. However, the so-called diffraction limit prevents detailed examination of these structures. Objects separated by less than 0.3 μm from one another cannot be resolved. Recently, different methods have been reported to overcome this limit, among them stochastic optical reconstruction microscopy (STORM).

The fluorescent signals from two nearby objects are superimposed by diffraction and imaged as a single feature. The ability to image individual probes separately means that their positions can be determined much more accurately to reconstruct the whole structure.

Here the cell structures are labelled with fluorescent dyes that respond with fluorescence emission upon stimulation by light of a certain wavelength. High resolution of approx. 0.02 μm can be achieved by switching off the majority of the dyes and leaving only a small number of them on. In this way, the light emitted by neighbouring dyes is no longer superimposed. This dye-switching is also controlled by light. The dyes left on can now be individually resolved and their positions can be determined by mathematical analysis to a very high accuracy of approx. 0.003 μm. Multiple repetition of this procedure supplies precise information about the location of all the dyes, thus permitting high-resolution reconstruction of the cell structures under investigation.

This method puts several demands on the microscope and the light sources used. Switching the respective dyes requires either different laser lines (excitation wavelength) or high light intensities or both. This can lead to problems in the investigation of living cells. Accordingly, the team headed by Heidelberg chemist Dr. Dirk-Peter Herten has replaced the switching of dyes via laser light with a light-independent process. To this end, the scientists adjusted a chemical probe for the detection of copper ions in such a way that the probe and its fluorescent properties can be used to mark cellular structures. If copper(II) binds to the probe, its fluorescence is suppressed. This binding of the copper(II) ion is reversible, thus restoring the fluorescence of the probe. The microscopic investigation of cell structures is therefore controlled by a reversible chemical reaction.

The scientists have called this method CHIRON – chemically improved resolution for optical nanoscopy. According to Dr. Herten, it means that microscopy methods like STORM can be simplified to such an extent that additional laser lines and high light intensities become superfluous. All that is required is for the probe to be located in a cellular environment to which minimal amounts of copper sulphate can be added, e.g. fixed cells. “This opens up new applications for high-resolution microscopy that were formerly impracticable due to technical restrictions,” says Dr. Herten. “Our probes can be used on many microscopes.”

For more information, go to www.bioquant.uni-heidelberg.de/research/groups/single_molecule_spectroscopy.html

Original publication
M. Schwering, A. Kiel, A. Kurz, K. Lymperopoulos, A. Sprödefeld, R. Krämer, D.-P. Herten: Far-Field Nanoscopy with Reversible Chemical Reactions / Hochauflösende Mikroskopie mit reversiblen chemischen Reaktionen. Angewandte Chemie International Edition, 15 February 2011, doi: 10.1002/anie.201006013

Contact:
Dr. Dirk-Peter Herten
Cluster of Excellence “CellNetworks”
Institute of Physical Chemistry
phone: +49 6221 5451220
dirk-peter.herten@urz.uni-hd.de

Communications and Marketing
Press Office, phone: +49 6221 542311
presse@rektorat.uni-heidelberg.de

Source: idw

www.uni-heidelberg.de

Share this post
  • Teile auf Facebook
  • Teile auf Twitter
  • Teile auf Google+
  • Teile per Mail
Sponsors
Related Posts
Archive

WITec refreshes its website www.witec.de

https://microscopy-news.com/wp-content/uploads/2017/01/fd45d6c858ed43477f37690b5a62b10e.jpg 135 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:45:102017-01-17 10:45:10WITec refreshes its website www.witec.de
Archive

Olympus Introduces New Microscope Objectives for Ultra-Deep Biological Imaging

https://microscopy-news.com/wp-content/uploads/2017/01/617bc24f81d3715820c3e91723ce1263.jpg 967 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:44:242017-01-17 10:44:24Olympus Introduces New Microscope Objectives for Ultra-Deep Biological Imaging
Archive

Andor launches KOMET 7 – The most advanced and powerful software solution for analysis, data management and presentation of comet assay samples

https://microscopy-news.com/wp-content/uploads/2017/01/516b76019f18322d8d3a9ddc96987741.jpg 300 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:44:242017-01-17 10:44:24Andor launches KOMET 7 – The most advanced and powerful software solution for analysis, data management and presentation of comet assay samples
Archive

Advanced Thin Films Enters Medical Laser Optics Market

https://microscopy-news.com/wp-content/uploads/2017/01/543ba9c56fe57e856dc7f252e2ebd3ba.jpg 466 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:43:462017-01-17 10:43:46Advanced Thin Films Enters Medical Laser Optics Market
Archive

Module transforms Raman microscopes into high throughput chemical analyzers

https://microscopy-news.com/wp-content/uploads/2017/01/7423894303b540804bd4b9ded95ac7a1.jpg 228 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:43:332017-01-17 10:43:33Module transforms Raman microscopes into high throughput chemical analyzers
Archive

Motic Microscopes launches the new BA410E for biomedical applications

https://microscopy-news.com/wp-content/uploads/2017/01/ca9ed333736eb38ce4abbffcee37be60.jpg 260 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:42:462017-01-17 10:42:46Motic Microscopes launches the new BA410E for biomedical applications
Archive

R&D 100 Award 2014 for ZEISS ELYRA P.1 with 3D-PALM

https://microscopy-news.com/wp-content/uploads/2017/01/2ed54dfbb5613dc8894327c8185309c1.jpg 175 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:42:442017-01-17 10:42:44R&D 100 Award 2014 for ZEISS ELYRA P.1 with 3D-PALM
Archive

Motic Microscopes presents the new AE31E for microbiology

https://microscopy-news.com/wp-content/uploads/2017/01/944a198042025732e6e8fb5a715d48c5.jpg 243 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:41:572017-01-17 10:41:57Motic Microscopes presents the new AE31E for microbiology
Archive

CRAIC Technologies Website: A Resource for UV-Visible-NIR and Raman Microspectroscopy

https://microscopy-news.com/wp-content/uploads/2017/01/3725b94ba62d8e2205f5f20b3cdee1ee.jpg 463 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:41:372017-01-17 10:41:37CRAIC Technologies Website: A Resource for UV-Visible-NIR and Raman Microspectroscopy
Archive

Imaging companies in Europe shoulder-to-shoulder with life scientists

https://microscopy-news.com/wp-content/uploads/2017/01/1affabb625a5c37519bafa89fedc162e.jpg 86 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:41:102017-01-17 10:41:10Imaging companies in Europe shoulder-to-shoulder with life scientists
Archive

Semrock Releases 20th Edition Master Catalog

https://microscopy-news.com/wp-content/uploads/2017/01/61e12630a94a26e3e618905ce9de549a.jpg 234 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:40:582017-01-17 10:40:58Semrock Releases 20th Edition Master Catalog
Archive

World’s Smallest Commercially Available Ball Screw!

https://microscopy-news.com/wp-content/uploads/2017/01/59d9386a4a7887c0e4775572b7f7511c.jpg 262 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:39:522017-01-17 10:39:52World’s Smallest Commercially Available Ball Screw!
Archive

NIST Announces New Competition for Advanced Manufacturing Planning Awards‏

https://microscopy-news.com/wp-content/uploads/2017/01/3486cdac57a29cec43d7506599bba9f1.jpg 188 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:39:482017-01-17 10:39:48NIST Announces New Competition for Advanced Manufacturing Planning Awards‏
Archive

XIMEA unveils Thunderbolt™ 2 high speed cameras with IMX174 and CMV20000

https://microscopy-news.com/wp-content/uploads/2017/01/341633931c01418c4fa8e0a90a8236d4.jpg 139 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:39:052017-01-17 10:39:05XIMEA unveils Thunderbolt™ 2 high speed cameras with IMX174 and CMV20000
Archive

New Blackfly® USB3 Vision™ CCD Camera Family Provides Easy Interface Transition

https://microscopy-news.com/wp-content/uploads/2017/01/082c31c09a15eff9e43b5c2fcf5f38cd.jpg 287 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:39:032017-01-17 10:39:03New Blackfly® USB3 Vision™ CCD Camera Family Provides Easy Interface Transition
Archive

Digital Surf and Image Metrology join forces

https://microscopy-news.com/wp-content/uploads/2017/01/3058648b93257cab2f2ca0eaad966c4b.jpg 174 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:38:262017-01-17 10:38:26Digital Surf and Image Metrology join forces
Archive

Bessel Beam Plane Illumination Microscopy Enables Fast 3D Volume Imaging

https://microscopy-news.com/wp-content/uploads/2017/01/70d120c9518cf134343f93d7a2adf4d3.jpg 181 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:38:142017-01-17 10:38:14Bessel Beam Plane Illumination Microscopy Enables Fast 3D Volume Imaging
Archive

Dolomite´s new microfluidic system showcases droplet merging at nanoliter sample scale

https://microscopy-news.com/wp-content/uploads/2017/01/22b06d4094468fc910406cd44d96ec1f.jpg 181 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:37:192017-01-17 10:37:19Dolomite´s new microfluidic system showcases droplet merging at nanoliter sample scale
Archive

Point Grey´s Latest Blackfly® GigE PoE Camera Features Popular High Sensitivity VGA CCD

https://microscopy-news.com/wp-content/uploads/2017/01/a5d0c77f7619812a129b2a4e6a9cb90b.jpg 234 350 otmaro https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png otmaro2017-01-17 10:36:522017-01-17 10:36:52Point Grey´s Latest Blackfly® GigE PoE Camera Features Popular High Sensitivity VGA CCD
Archive

Point Grey Launches New Grasshopper®3 Family of High Resolution GigE Vision™ PoE Cameras

https://microscopy-news.com/wp-content/uploads/2017/01/a77db128b10a613c26b9e4dbea5dae02.jpg 220 350 Ottermedia https://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.png Ottermedia2017-01-17 10:36:392017-01-17 10:36:39Point Grey Launches New Grasshopper®3 Family of High Resolution GigE Vision™ PoE Cameras
ZurückWeiter

Sponsors

Follow us

Products

  • Upright Microscopes
  • Inverted Microscopes
  • Stereo Microscopes
  • Electron Microscopes
  • Imaging Cameras
  • Imaging Software
  • Systems
  • Medical/Surgical Solutions
  • Accessoires

Navigation

  • Microscopy News
  • MediaKit / Sponsoring
  • Contact
  • Privacy
  • Imprint

Contact

Microscopy News Portal
Am Ginster 6
21409 Oerzen, Germany

Email:
info@microscopy-news.com

Search

© Copyright - Microscopy-News.com 2017 | Germany
  • Facebook
  • Twitter
  • Mail
  • Contact
  • Privacy
  • Imprint
TILL Photonics introduces Upright 2-Photon Microscope New Scanning Probe Microscopy Book Features Articles by Asylum Research Scientists...
We use cookies to improve your experience with our site.OKLearn more
Nach oben scrollen