Andor sCMOS Camera´s Speed, Resolution and Sensitivity key to Brain-wide 3D Imaging
High-speed imaging of neuronal activity across entire Brain for the first time
26th February 2014, Belfast, UK: Although the entire nervous system connectivity map of the nematode model organism, Caenorhabditis elegans, has been known for more than 25 years, it has not been possible to predict all functional connections underlying behaviour. Now, the Vaziri and Zimmer research groups in Vienna, Austria, have demonstrated a high-speed imaging technique that allows neuronal activity across the entire brain to be recorded simultaneously.
Publishing in Nature Methods, Professor Alipasha Vaziri ascribes two key breakthroughs for their success in being the first to implement brain-wide imaging. “By choosing the Andor Neo 5.5 megapixel sCMOS camera, we were able to image the entire volume associated with the worm’s brain (75 x 75 x 30 μm) four to six times per second using wide-field temporal focusing (WF-TeFo) two-photon microscopy. Plus, we used a novel Calcium-indicator that visualises the activity of a neuron by increasing its fluorescence and permits unambiguous discrimination of individual neurons within the densely packed head ganglia.
Dr Manuel Zimmer backs this up, saying “The Andor Neo camera was one of the only two sCMOS cameras then available and our demo-trial convinced us that it provided the fastest speed while retaining high sensitivity and a wide dynamic range. We also chose Andor over its competitors because of its capability to integrate easily into our home-made hardware control interface, as well as their good customer and technical support service.”
WF-TeFo microscopy allows users to independently control the axial and lateral confinement of the excitation area while taking advantage of the high depth penetration and low scattering properties of two-photon excitation. For their volumetric imaging, the group scanned an excitation area of approx. 60 μm in diameter with an axial confinement of approx. 1.9 μm in the axial direction only to speed up the volume acquisition time. A volume acquisition rate approaching 13 Hz was achievable but imaging was performed at speeds of 4-6 Hz, corresponding to approx. 5.6 megavoxels per second, achieving a lateral spatial resolution of 0.285 μm.
“The value of this work to the neuroscience community is evident in the attention it attracted online, ranking second overall for Nature Methods,” says Orla Hanrahan of Andor. “Because their imaging methodology can also be applied straightforwardly to other organisms, or more general imaging tasks, and since their novel calcium reporter is a very powerful tool that can be used by all neuroscientists working on C. elegans, their technique has already had a large impact. If a lab-on-a-chip device is deployed for stimulus delivery in the future, this method provides an enabling platform for establishing functional maps of neuronal networks.”
Unlike previous CMOS or CCD technologies, the Andor Neo 5.5 sets radical new benchmarks in its unique ability to simultaneously deliver highest specifications in sensitivity, resolution, speed, dynamic range and field-of-view. The camera is based around a large 5.5 megapixel sensor with 6.5 µm pixels and a 22 mm diameter and is capable of 30 fps sustained or up to 100 fps burst to the 4 GB internal memory, making it ideal for applications in cell microscopy, astronomy, digital pathology, and high content screening. Built on a -40°C vacuum-cooled platform, the extremely low darkcurrent and 1 e- read noise means Neo 5.5 is suited to a range of exposure conditions. The Rolling and Global shutter flexibility further enhances application flexibility, Global shutter in particular offering an ideal means to simply and efficiently synchronize the Neo with other ‘moving’ devices, such as stages or light switching sources, and eliminating the possibility of spatial distortion when imaging fast moving objects.
About the Vaziri and Zimmer Groups
The Vaziri group’s research focuses on design and exploration of novel microscopy methods with a particular interest in neuro-scientific applications, such as fast volumetric imaging of neuronal activity. The Zimmer group focuses on neural circuits involved in chemo-sensing in C. elegans. In their recent Nature Methods paper, these two research directions were joined to deliver an exciting platform for demonstrating the capabilities and power of modern microscopy techniques.
The image above is available for download. Either click on the image or contact John Waite at Catalyst Communications.
Tina Schrödel, Robert Prevedel, Karin Aumayr, Manuel Zimmer and Alipasha Vaziri “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light” Nature Methods Vol. 10, 1013-1020 (2013)
Andor is a global leader in the pioneering and manufacturing of high performance scientific imaging cameras, spectroscopy solutions and microscopy systems for research and OEM markets. Andor has been innovating the photonics industry for over 20 years and continues to set the standard for high performance light measuring solutions, enabling its customers to break new ground by performing light measurements previously considered impossible. Andor’s digital cameras, are allowing scientists around the world to measure light down to a single photon and capture events occurring within 1 billionth of a second.
Andor now has over 400 staff across 16 offices worldwide, distributing products to over 10,000 customers in 55 countries. Andor’s products are used in a wide range of applications including medical research to further the understanding of heart disease, cancer and neuronal diseases such as Alzheimer’s and Parkinson’s disease. Andor also has applications for forensic science and astronomy. Through continuous dialogue with customers and strong teamwork, Andor continues to innovate ground-breaking products that improve the world in which we live.
About Oxford Instruments plc
Oxford Instruments designs, supplies and supports high-technology tools and systems with a focus on research and industrial applications. Innovation has been the driving force behind Oxford Instruments’ growth and success for over 50 years, and its strategy is to effect the successful commercialisation of these ideas by bringing them to market in a timely and customer-focused fashion.
The first technology business to be spun out from Oxford University, Oxford Instruments is now a global company with over 2300 staff worldwide and is listed on the FTSE250 index of the London Stock Exchange (OXIG). Its objective is to be the leading provider of new generation tools and systems for the research and industrial sectors with a focus on nanotechnology. Its key market sectors include nano-fabrication and nano-materials. The company’s strategy is to expand the business into the life sciences arena, where nanotechnology and biotechnology intersect.
This involves the combination of core technologies in areas such as low temperature, high magnetic field and ultra high vacuum environments; Nuclear Magnetic Resonance; x-ray, electron, laser and optical based metrology; atomic force microscopy; optical imaging; advanced growth, deposition and etching.
Oxford Instruments aims to pursue responsible development and deeper understanding of our world through science and technology. Its products, expertise, and ideas address global issues such as energy, environment, security and health.
Andor Technology plc.
7 Millennium Way
Springvale Business Park
Belfast BT12 7AL
+44 (0) 28 9027 0812
https://microscopy-news.com/wp-content/uploads/2017/01/516b76019f18322d8d3a9ddc96987741.jpg300350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:44:242017-01-17 10:44:24Andor launches KOMET 7 – The most advanced and powerful software solution for analysis, data management and presentation of comet assay samples
https://microscopy-news.com/wp-content/uploads/2017/01/543ba9c56fe57e856dc7f252e2ebd3ba.jpg466350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:43:462017-01-17 10:43:46Advanced Thin Films Enters Medical Laser Optics Market
https://microscopy-news.com/wp-content/uploads/2017/01/7423894303b540804bd4b9ded95ac7a1.jpg228350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:43:332017-01-17 10:43:33Module transforms Raman microscopes into high throughput chemical analyzers
https://microscopy-news.com/wp-content/uploads/2017/01/ca9ed333736eb38ce4abbffcee37be60.jpg260350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:42:462017-01-17 10:42:46Motic Microscopes launches the new BA410E for biomedical applications
https://microscopy-news.com/wp-content/uploads/2017/01/2ed54dfbb5613dc8894327c8185309c1.jpg175350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:42:442017-01-17 10:42:44R&D 100 Award 2014 for ZEISS ELYRA P.1 with 3D-PALM
https://microscopy-news.com/wp-content/uploads/2017/01/944a198042025732e6e8fb5a715d48c5.jpg243350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:41:572017-01-17 10:41:57Motic Microscopes presents the new AE31E for microbiology
https://microscopy-news.com/wp-content/uploads/2017/01/3725b94ba62d8e2205f5f20b3cdee1ee.jpg463350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:41:372017-01-17 10:41:37CRAIC Technologies Website: A Resource for UV-Visible-NIR and Raman Microspectroscopy
https://microscopy-news.com/wp-content/uploads/2017/01/1affabb625a5c37519bafa89fedc162e.jpg86350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:41:102017-01-17 10:41:10Imaging companies in Europe shoulder-to-shoulder with life scientists
https://microscopy-news.com/wp-content/uploads/2017/01/341633931c01418c4fa8e0a90a8236d4.jpg139350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:39:052017-01-17 10:39:05XIMEA unveils Thunderbolt™ 2 high speed cameras with IMX174 and CMV20000
https://microscopy-news.com/wp-content/uploads/2017/01/22b06d4094468fc910406cd44d96ec1f.jpg181350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:37:192017-01-17 10:37:19Dolomite´s new microfluidic system showcases droplet merging at nanoliter sample scale
https://microscopy-news.com/wp-content/uploads/2017/01/a5d0c77f7619812a129b2a4e6a9cb90b.jpg234350otmarohttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngotmaro2017-01-17 10:36:522017-01-17 10:36:52Point Grey´s Latest Blackfly® GigE PoE Camera Features Popular High Sensitivity VGA CCD
https://microscopy-news.com/wp-content/uploads/2017/01/a77db128b10a613c26b9e4dbea5dae02.jpg220350Ottermediahttps://microscopy-news.com/wp-content/uploads/2017/01/Microscopy-news_logo_microscope_news.pngOttermedia2017-01-17 10:36:392017-01-17 10:36:39Point Grey Launches New Grasshopper®3 Family of High Resolution GigE Vision™ PoE Cameras