New multifocal plane microscopy method described today at 55th Biophysical Society Meeting in Baltimore
WASHINGTON, D.C. (March 8, 2011) — Researchers at the University of Texas Southwestern Medical Center and the University of Texas at Dallas are reporting today at the 55th Annual Biophysical Society Annual Meeting in Baltimore, MD how they are using a novel 3D cell imaging method for studying the complex spatial-temporal dynamics of protein transport, providing a solution to this fundamental problem in cell biology.
Graphic illustrating the strengths of strengths of multifocal plane microscopy over conventional microscopy techniques.
According to the authors of the study, imaging such highly dynamic processes in the cell and in 3D poses major technical challenges in a complex cell monolayer due to cell-to-cell variations in thickness and temporal properties of protein transport. Previous imaging techniques were slow and suffered from poor z-localization/3D-tracking capability.
Using a combination of multifocal plane microscopy (MUM) and nanodot labeling technology, the researchers were able to label single molecules in live cells and track their movement and their interaction with other molecules in a thick cell sample for extended periods of time.
Sripad Ram, the lead author of the study, explains that the main reason he and his colleagues developed these imaging techniques is to track the movement of therapeutic antibodies, which are engineered in their lab. “We want to know where these antibodies go and what they do once they enter the body,” says Ram.
He adds that “current microscopy technologies are limited in that you can only image a single focal plane at any given time. If you want to image in three dimensions, you can only do so sequentially, but you end up imaging at the wrong place and at the wrong time thereby missing events over time….what we needed was a technology that could simultaneously image a sample across multiple planes, and that is what multifocal plane microscopy is all about.”
The presentation, “3D SINGLE MOLECULE TRACKING IN THICK CELLULAR SAMPLES USING MULTIFOCAL PLANE MICROSCOPY” by Sripad Ram, E. Sally Ward, and Raimund J. Ober is at 11:00 am. on Tuesday, March 8, 2011 in Ballroom IV of the Baltimore Convention Center. ABSTRACT: http://tinyurl.com/4zzt6ys
This work was funded by the National Institutes of Health and the National Multiple Sclerosis Society.
MORE MEETING INFORMATION
Each year, the Biophysical Society Annual Meeting brings together more than 6,000 scientists and hosts more than 4,000 poster presentations, 200 exhibits, and more than 20 symposia. The largest meeting of its type in the world, the Biophysical Society Annual Meeting retains its small-meeting flavor through its subgroup meetings, platform sessions, social activities, and committee programs.
The Biophysical Society, founded in 1956, is a professional, scientific society established to encourage development and dissemination of knowledge in biophysics. The society promotes growth in this expanding field through its annual meeting, monthly journal, and committee and outreach activities. Its over 9,000 members are located throughout the U.S. and the world, where they teach and conduct research in colleges, universities, laboratories, government agencies, and industry. For more information on the society or the 2011 Annual Meeting, visit www.biophysics.org Source: American Institute of Physics
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